Hairless canary seeds (Phalaris canariensis L.) as a potential source of antioxidant, antihypertensive, antidiabetic, and antiobesity biopeptides

Abstract Rising consumer concerns with synthetic drugs to treat non-communicable diseases (NCDs) have promoted a shift towards using natural biological active constituents that offer similar health benefits. Hairless canary seed ( Phalaris canariensis L ) is an emerging crop traditionally used in Mexico NCDs. Peptides liberated during simulated digestion of protein are believed be responsible for their activity; however, no studies shown the effect controlled hydrolysis commercial proteases on protein’s activity. Therefore, this study aimed explore vitro antihypertensive, antidiabetic, and anti-obesity activity peptides derived from proteolysis Alcalase®. Protein fractions were primarily composed prolamins (54.07 ± 1.8%), glutelins (32.19 3.18%), globulins (5.97 0.52%) albumins 0.52%). The < 3 kDa 3–10 peptide showed highest inhibition capacity p 0.05) angiotensin-converting enzyme (IC 50 = 0.028–0.032 mg/mL) lipase 2.15–2.27 mg/mL), α-glucosidase 0.82–1.15 dipeptidyl-peptidase-IV 1.27–1.60 mg/mL). Additionally, these high antioxidant against DPPH (134.22–150.66 μmol TE/mg) ABTS (520.92–813.33 TE/mg). These results provide insight into potential development functional foods enzymatic proteins treating hypertension, type-2 diabetes, obesity.